生物大分子重点实验室

1998 - 2003  美国伊利诺伊大学香槟分校（UIUC），博士

2003 - 2007  美国斯克利普斯研究所（The Scripps Research Institute），博士后

2007 - 至今   中国科学院生物物理研究所，研究员

2015 - 至今   中国科学院大学，岗位教授

　1. 蛋白质及RNA定点标记化学生物学，应用于蛋白质核酸药物修饰和优化、光镜及电镜成像。

　2. 酶化学生物学及合成生物学，应用于致病微生物防控及人工光合作用、碳中和等领域。

　3. G-蛋白偶联受体机理研究及合成生物学研究, 应用于主动健康及疾病诊疗。

　　 主要在研项目：

     部分已结题项目：

课题组学生参加学术活动及获奖情况：

王江云课题组的研究生获得了丰富的研究成果，并积极参加学术活动。例如，王江云课题组于2019年组织酶化学生物学研讨会, 研究生和2018年诺贝尔化学奖获得者Frances Arnold等著名教授展开深入交流。

王江云课题组已有多名学生获得国家奖学金、中国科学院院长奖学金、光华奖学金、朱李月华优秀博士生奖学金、生物物理所所长奖学金、中国科学院院长奖学金优秀奖、中国科技大学优秀毕业生等。

实验室的快乐生活：

2021年实验室秋游

2021年10月实验室聚餐

毕业学生情况：

在顺利获得学位之后，各位同学带着导师的期待，继续迎接着人生的一个又一个的辉煌！就业单位有：

1. 国外高校或研究所：美国耶鲁大学、美国康奈尔大学、Scripps 研究所、马萨诸塞大学阿默斯特分校、哥伦比亚大学等；

2. 国内高校、研究所或企事业单位：如中国科学院化学研究所、深圳湾实验室 (独立PI岗位）、北京市理化分析测试中心等；

3. 国内外公司：IDG投资、药明康德、晶泰科技、贝瑞基因、恒瑞医药等。

课题组近5年取得的重要研究进展：

1. 2020年 王江云课题组与合作者在G蛋白偶联受体信号转导偏好性的机制研究中取得新进展

http://ibp.cas.cn/kyjz/2020kyjz2/202009/t20200930_5710016.html

2. 2021年 王江云/钟芳锐、吴钰周教授课题组在生物催化交叉偶联-光驱动卤代芳烃羟化脱卤酶的设计方面获得重要进展

http://ibp.cas.cn/kyjz/2021kyjz/202101/t20210108_5853979.html

3. 2021年 王江云/李运良/庄巍课题组在基因编码二维红外探针研究酶活性中心柔性方面获得重要进展

http://ibp.cas.cn/kyjz/2021kyjz/202103/t20210301_5963635.html

4. 2021年 中国科学院生物物理研究所王江云研究团队和合作者在G蛋白偶联受体磷酸化编码机制研究中取得新进展

http://ibp.cas.cn/kyjz/2021kyjz/202105/t20210531_6046213.html

5. 2021年 王江云/田长麟课题组在光催化CO2还原酶的理性设计方面获得重要进展

http://ibp.cas.cn/kyjz/2021kyjz/202104/t20210425_5996822.html

6. 2021年 王江云研究团队和合作者在smFRET 检测GPCR 调控下游蛋白arrestin的构象分布研究中取得重大进展

http://ibp.cas.cn/kyjz/2021kyjz/202106/t20210608_6080508.html

7. 2021年 王江云/夏安东/于龙江课题组在高效光致电子转移光敏蛋白质的理性设计方面获得重要进展

http://ibp.cas.cn/kyjz/2021kyjz/202106/t20210616_6108414.html

8. 2021年，王江云/刘志杰/华甜课题组在GPCR别构调节机制的研究方面获得重要进展

http://ibp.cas.cn/kyjz/2021kyjz/202110/t20211008_6219306.html

9. 2022年，王江云/陈永湘课题组在遗传编码膦配体及人工金属蛋白（酶）设计研究中取得重要进展

http://ibp.cas.cn/kyjz/2022kyjz/202211/t20221124_6554199.html

10. 2023年，王江云/董敏研究组实现SAM自由基酶的光酶还原

http://ibp.cas.cn/kyjz/zxdt/202305/t20230506_6749693.html

11. 2023年，王江云/钟芳锐课题组在基因改造的光卤化酶催化芳基卤化物的全细胞氢化/氘化获得重要进展

http://ibp.cas.cn/kyjz/zxdt/202304/t20230406_6727752.html

12. 2024年，王江云研究组在大麻素受体配体识别和G蛋白选择性机制研究方面取得重要进展

http://ibp.cas.cn/kyjz/zxdt/202402/t20240205_6985875.html

13. 2024年，王江云研究组在揭示肠道菌群发酵的短链脂肪酸感知机制方面取得重要进展

http://ibp.cas.cn/kyjz/zxdt/202401/t20240110_6951562.html

14. 2024年，王江云研究组在琥珀酸感知机制方面取得重要进展

http://ibp.cas.cn/kyjz/zxdt/202405/t20240516_7162787.html

1. Wang T, Tang W, Zhu X, Lv Z, Chen J, Li Y, Sun X, Lv H, Gu Q, Li F*, Wang J*. Molecular activation and G protein coupling selectivity of human succinate receptor SUCR1. Cell Research. 2024, 0:1-4, DOI: 10.1038/s41422-024-00968-7

2. Wang T, Tang W, Zhao Z, Zhao R, Lv Z, Guo X, Gu Q, Liu B, Lv H, Chen J, Zhang K, Li F*, Wang J*. Fenofibrate Recognition and Gq Protein CouplingMechanisms of the Human Cannabinoid Receptor CB1. Adv. Sci. 2024, 2306311，DOI: 10.1002/advs.202306311

3. Li F, Tai L, Sun X, Lv Z, Tang W, Wang T, Zhao Z, Gong D, Ma S, Tang S, Gu Q, Zhu X, Yu M, Liu X, Wang J*. Molecular recognition and activation mechanism of short-chain fatty acid receptors FFAR2/3. Cell Research. 2024 ,34, 323-326, DOI: 10.1038/s41422-023-00914-z

4. Fu Y, Liu X, Xia Y, Guo X, Guo J, Zhang J, Zhao W, Wu Y, Wang J*, and Zhong F*. Whole-cell-catalyzed hydrogenation/deuteration of aryl halides with a genetically repurposed photodehalogenases, Chem. 2023, 9, 1897-1909, DOI: 10.1016/j.chempr.2023.03.006

5. Huang R, Zhi N, Yu L, Li Y, Wu X, He J, Zhu H, Qiao J, Liu, X, Tian C, Wang J*, and Dong M*. Genetically Encoded Photosensitizer Protein Reduces Iron?Sulfur Clusters of Radical SAM Enzymes, ACS Catal. 2023, 13, 1240?1245, DOI: 10.1021/acscatal.2c05143

6. Xu C, Zou Q, Tian J, Li M, Xing B, Gong J, Wang J*, Huo Y*, Guo S*. Simplified Construction of Engineered Bacillus subtilis Host for Improved Expression of Proteins Harboring Noncanonical Amino Acids, ACS Synth. Biol. 2023, 12, 583?595, DOI: 10.1021/acssynbio.2c00604

7. Duan H, Hu C, Li Y, Wang S, Xia Y, Liu X*, Wang J*, Chen Y*. Genetically Encoded Phosphine Ligand for Metalloprotein Design, J. Am. Chem. Soc. 2022, 144, 22831?22837, DOI:10.1021/jacs.2c09683

8. Liu X, Liu P, Li H, Xu Z, Jia L, Xia Y, Yu M, Tang W, Zhu X, Chen C, Zhang Y, Nango E, Tanaka R, Luo F, Kato K, Nakajima Y, Kishi S, Yu H, Matsubara N, Owada S, Tono K, Iwata S, Yu L*, Shen J*, Wang J*. Excited-state intermediates in a designer protein encoding a phototrigger caught by an X-ray free-electron laser. Nat Chem. 2022, 14(9):1054-1060. DOI: 10.1038/s41557-022-00992-3.

9. Wang P, Zhang G, Xu Z, Chen Z, Liu X, Wang C, Zheng C, Wang J*, Zhang H*, Yan A*. Whole-cell FRET monitoring of transcription factor activities enables functional annotation of signal transduction systems in living bacteria. J Biol Chem. 2022, 298(8):102258. DOI: 10.1016/j.jbc.2022.102258.

10. Yang K, Yu M, Zhu X, Xia Y, Li F, Li Y, Liu X*, Wang J*. Genetic Incorporation of Fluorescent Amino Acid into Fatty Acid Binding Protein for Fatty Acid Detection. Journal of Molecular Biology. 2022, 434(8): 167498. DOI: 10.1016/j.jmb.2022.167498

11. Han Y, Guo X, Zhang T, Wang J*, Ye K*. Development of an RNA-protein crosslinker to capture protein interactions with diverse RNA structures in cells. RNA. 2022, 28(3):390-399. DOI: 10.1261/rna.078896.121.

12. Cheng R, Lai R, Peng C, Lopez J, Li Z, Naowarojna N, Li K, Wong C, Lee N, Whelan SA, Qiao L, Grinstaff M, Wang J, Cui Q*, and Liu P*. Implications for an Imidazole-2-yl Carbene Intermediate in the Rhodanase-Catalyzed C-S Bond Formation Reaction of Anaerobic Ergothioneine Biosynthesis. ACS Catal. 2021, 11, 6, 3319-3334. DOI: https://doi.org/10.1021/acscatal.0c04886

13. Wang X, Liu D, Shen L, Li F, Li Y, Yang L, Xu T, Tao H, Yao D, Wu L, Hirata K, Bohn L, Makriyannis A, Liu X, Hua T*, Liu Z*, and Wang J*. A Genetically Encoded F-19 NMR Probe Reveals the Allosteric Modulation Mechanism of Cannabinoid Receptor 1. J. Am. Chem. Soc. 2021, 143, 40, 16320-16325. DOI: 10.1021/jacs.1c06847

14. Zheng D, Tao M, Yu L, Liu X*, Xia A*, Wang J*. Ultrafast Photoinduced Electron Transfer in a Photosensitizer Protein. CCS Chem. 2021, 3, 1580-1586. DOI: 10.31635/ccschem.021.202100823

15. Han M, He Q, Yang M, Chen C, Yao Y, Liu X, Wang Y, Zhu Z, Zhu K, Qu C, Yang F, Hu C, Guo X, Zhang D, Chen C*, Sun J* and Wang J*. Single-molecule FRET and conformational analysis of beta-arrestin-1 through genetic code expansion and Se-Click reaction. Chemical Science, 2021,12, 9114-9123, DOI: 10.1039/D1SC02653D

16. Wu E, Guo X, Teng X, Zhang R, Li F, Cui Y, Zhang D, Liu Q, Luo J*, Wang J*, Chen R*. Discovery of Plasma Membrane-Associated RNAs through APEX-seq. Cell Biochem Biophys. 2021, 79, 905-917. DOI: https://doi.org/10.1007/s12013-021-00991-0

17. Wang Y, Liu P, Chang J, Xu Y*, Wang J*. Site-specific selenocysteine incorporation into proteins by genetic engineering. ChemBioChem. 2021, 22, 2918 -2924. DOI: https://doi.org/10.1002/cbic.202100124

18. He Q, Xiao P, Huang S, Jia Y, Zhu Z, Lin J, Yang F, Tao X, Zhao R, Gao F, Niu X, Xiao K, Wang J*, Jin C*, Sun J*& Yu X*. Structural studies of phosphorylation-dependent interactions between the V2R receptor and arrestin-2. Nat Commun 2021,12, 2396. DOI: https://doi.org/10.1038/s41467-021-22731-x

19. Wang L, Zhang J , Han M, Zhang L, Chen C, Huang A, Xie R, Wang G, Zhu J, Wang Y, Liu X*, Zhuang W*, Li Y*, Wang J*. A Genetically Encoded Two-Dimensional Infrared Probe for Enzyme Active-Site Dynamics. Angew Chem Int Ed Engl. 2021, 60, 11143 -11147. DOI: https://doi.org/10.1002/anie.202016880

20. Fu Y, Huang J, Wu Y*, Liu X*, Zhong F*, and Wang J*. Biocatalytic Cross-Coupling of Aryl Halides with a Genetically Engineered Photosensitizer Artificial Dehalogenase. J. Am. Chem. Soc. 2021, 143, 2, 617-622. DOI: 10.1021/jacs.0c10882

21. Kang F, Yu L, Xia Y, Yu M, Xia L, Wang Y, Yang L, Wang T, Gong W, Tian C*, Liu X*, and Wang J*. Rational Design of a Miniature Photocatalytic CO2-Reducing Enzyme. ACS Catal. 2021, 11, 9, 5628-5635. DOI: 10.1021/acscatal.1c00287

22. An X, Chen C, Wang T, Huang A, Zhang D, Han M, Wang J*. Genetic Incorporation of Selenotyrosine Significantly Improves Enzymatic Activity of Agrobacterium radiobacter Phosphotriesterase. ChemBioChem. 2021, 22, 2535- 2539, DOI: 10.1002/cbic.202000460

23. Zheng Z, Guo X, Yu M, Wang X, Lu H, Li F, Wang J*. Identification of Human IDO1 Enzyme Activity by Using Genetically Encoded Nitrotyrosine. ChemBioChem 2020 , 21(11):1593 -1596. DOI: 10.1002/cbic.201900735

24. Liu Q, He Q, Lyu X, Yang F, Zhu Z, Xiao P, Yang Z, Zhang F, Yang Z, Wang X, Sun P, Wang Q, Qu C, Gong Z, Lin J, Xu Z, Song S, Huang S, Guo S, Han M, Zhu K, Chen X, Kahsai A., Xiao K, Kong W, Li F, Ruan K, Li Z, Yu X, Niu X, Jin C, Wang J* & Sun J*. DeSiphering receptor core-induced and ligand-dependent conformational changes in arrestin via genetic encoded trimethylsilyl 1H-NMR probe. Nature Communications. 2020, 11, 4857. DOI: https://doi.org/10.1038/s41467-020-18433-5

25. Hu C, Liu X, Wang J*. Electrostatics affect the glow. Science. 2020, 367(6473):26. DOI: 10.1126/science.aba0571.

26. Zheng D, Zhang Y, Liu X, Wang J*. Coupling natural systems with synthetic chemistry for light-driven enzymatic biocatalysis. Photosynth Res. 2020, 143(2):221-231. DOI: 10.1007/s11120-019-00660-7

27. Huang S, Yang F, Cai B, He Q, Liu Q, Qu C, Han M, Kong W, Jia YL, Li F, Yu X, Sun J*, Wang J*. Genetically Encoded Fluorescent Amino Acid for Monitoring Protein Interactions through FRET. Anal Chem. 2019, 91(23):14936-14942. DOI: 10.1021/acs.analchem.9b03305.

28. Xia L, Han M, Zhou L, Huang A, Yang Z, Wang T, Li F, Yu L, Tian C, Zang Z, Yang QZ, Liu C, Hong W, Lu Y, Alfonta L, Wang J*. S-Click Reaction for Isotropic Orientation of Oxidases on Electrodes to Promote Electron Transfer at Low Potentials. Angew Chem Int Ed Engl. 2019, 58(46):16480-16484.DOI: 10.1002/anie.201909203.

29. Li K, Hou X, Li R, Bi W, Yang F, Chen X, Xiao P, Liu T, Lu T, Zhou Y, Tian Z, Shen Y, Zhang Y, Wang J, Fang H, Sun J*, Yu X*. Identification and structure-function analyses of an allosteric inhibitor of the tyrosine phosphatase PTPN22. J Biol Chem. 2019, 294(21):8653-8663. DOI: 10.1074/jbc.RA118.007129.

30. Yu Y, Liu X, Wang J*. Expansion of Redox Chemistry in Designer Metalloenzymes. Acc Chem Res. 2019,52(3):557-565. DOI: 10.1021/acs.accounts.8b00627.

31. Wang L, Chen X, Guo X, Li J, Liu Q, Kang F, Wang X, Hu C, Liu H, Gong W, Zhuang W, Liu X*, Wang J*. Significant expansion and red-shifting of fluorescent protein chromophore determined through computational design and genetic code expansion. Biophys Rep. 2018, 4(5):273-285. DOI: 10.1007/s41048-018-0073-z.

32. Liu X, Kang F, Hu C, Wang L, Xu Z, Zheng D, Gong W, Lu Y, Ma Y, Wang J*. A genetically encoded photosensitizer protein facilitates the rational design of a miniature photocatalytic CO2-reducing enzyme. Nat Chem. 2018, 10 (12):1201-1206. DOI: 10.1038/s41557-018-0150-4.

33. Mu Z, Zou Z, Yang Y, Wang W, Xu Y, Huang J, Cai R, Liu Y, Mo Y, Wang B, Dang Y, Li Y, Liu Y, Jiang Y, Tan Q, Liu X, Hu C, Li H, Wei S, Lou C, Yu Y, Wang J*. A genetically engineered Escherichia coli that senses and degrades tetracycline antibiotic residue. Synth Syst Biotechnol. 2018, 3(3):196-203. DOI: 10.1016/j.synbio.2018.05.001.

34. Dong J, Li F, Gao F, Wei J, Lin Y, Zhang Y, Lou J, Liu G, Dong Y, Liu L, Liu H, Wang J, Gong W*. Structure of tRNA-Modifying Enzyme TiaS and Motions of Its Substrate Binding Zinc Ribbon. J Mol Biol. 2018, 430(21):4183-4194. DOI: 10.1016/j.jmb.2018.08.015.

35. Yang F, Xiao P, Qu CX, Liu Q, Wang L, Liu Z, He Q, Liu C, Xu J, Li R, Li M, Li Q, Guo X, Yang Z, He D, Yi F, Ruan K, Shen Y, Yu X, Sun J*, Wang J*. Allosteric mechanisms underlie GPCR signaling to SH3-domain proteins through arrestin. Nat Chem Biol. 2018, 14(9):876-886. DOI: 10.1038/s41589-018-0115-3.

36. Zhang J, Wang L, Zhang J, Zhu J, Pan X, Cui Z, Wang J*, Fang W*, Li Y*. Identifying and Modulating Accidental Fermi Resonance: 2D IR and DFT Study of 4-Azido-l-phenylalanine. J Phys Chem B. 2018, 122(34):8122-8133. DOI: 10.1021/acs.jpcb.8b03887.

37. Li J, Griffith WP, Davis I, Shin I, Wang J, Li F, Wang Y, Wherritt DJ, Liu A*. Cleavage of a carbon-fluorine bond by an engineered cysteine dioxygenase. Nat Chem Biol. 2018, 14(9):853-860. DOI: 10.1038/s41589-018-0085-5.

38. Zhang F, Zhou Q, Yang G, An L, Li F*, Wang J*. A genetically encoded 19F NMR probe for lysine acetylation. Chem Commun (Camb). 2018, 54(31):3879-3882. DOI: 10.1039/c7cc09825a.

39. Chen L, Naowarojna N, Song H, Wang S, Wang J, Deng Z, Zhao C, Liu P*. Use of a Tyrosine Analogue To Modulate the Two Activities of a Nonheme Iron Enzyme OvoA in Ovothiol Biosynthesis, Cysteine Oxidation versus Oxidative C-S Bond Formation. J Am Chem Soc. 2018, 140(13):4604-4612. DOI: 10.1021/jacs.7b13628.

40. Yu Y, Hu C, Xia L, and Wang J*. Artificial Metalloenzyme Design with Unnatural Amino Acids and Non-Native Cofactors. ACS Catal. 2018, 8 (3):1851-1863. DOI:  10.1021/acscatal.7b03754

41. Pan Y, Zhang H, Zheng Y, Zhou J, Yuan J, Yu Y, Wang J*. Resveratrol Exerts Antioxidant Effects by Activating SIRT2 To Deacetylate Prx1. Biochemistry. 2017, 56(48):6325-6328. DOI: 10.1021/acs.biochem.7b00859.

42. Lu J, Zhang H, Chen X, Zou Y, Li J, Wang L, Wu M, Zang J, Yu Y, Zhuang W*, Xia Q*, Wang J*. A small molecule activator of SIRT3 promotes deacetylation and activation of manganese superoxide dismutase. Free Radic Biol Med. 2017, 112:287-297. DOI: 10.1016/j.freeradbiomed.2017.07.012.

43. Hu C, Yu Y, Wang J*. Improving artificial metalloenzymes' activity by optimizing electron transfer. Chem Commun (Camb). 2017, 53(30):4173-4186. DOI: 10.1039/c6cc09921a.

44. Yang F, Yu X, Liu C, Qu C, Gong Z, Liu H, Li F, Wang H, He D, Yi F, Song C, Tian C, Xiao K, Wang J*, Sun J*. Phospho-selective mechanisms of arrestin conformations and functions revealed by unnatural amino acid incorporation and ¹⁹F-NMR. Nat Commun. 2015, 6:8202. DOI: 10.1038/ncomms9202.

45. Yu Y, Zhou Q, Wang L, Liu X, Zhang W, Hu M, Dong J, Li J, Lv X, Ouyang H, Li H, Gao F, Gong W, Lu Y*, Wang J*. Significant Improvement of Oxidase Activity through the Genetic Incorporation of a Redox-active Unnatural Amino Acid. Chem Sci. 2015, 6(7): 3881-3885. DOI: 10.1039/C5SC01126D

46. Yu Y, Cui C, Liu X, Petrik ID, Wang J*, Lu Y*. A Designed Metalloenzyme Achieving the Catalytic Rate of a Native Enzyme. J Am Chem Soc. 2015, 137(36):11570-11573. DOI: 10.1021/jacs.5b07119.

47. Yu Y, Hu C, Liu X, Wang J*. Synthetic Model of the Oxygen-Evolving Center: Photosystem II under the Spotlight. Chembiochem. 2015, 16(14):1981-1983. doi: 10.1002/cbic.201500302.

48. He T, Gershenson A, Eyles SJ, Lee YJ, Liu WR, Wang J, Gao J, Ro, Roberts MF*. Fluorinated Aromatic Amino Acids Distinguish Cation-π Interactions from Membrane Insertion. J Biol Chem. 2015, 290(31):19334-19342. DOI: 10.1074/jbc.M115.668343.

49. Lv X, Yu Y, Zhou M, Hu C, Gao F, Li J, Liu X, Deng K, Zheng P, Gong W, Xia A*, Wang J*. Ultrafast photoinduced electron transfer in green fluorescent protein bearing a genetically encoded electron acceptor. J Am Chem Soc. 2015, 137(23):7270-7273. DOI: 10.1021/jacs.5b03652.

50. Wang T, Zhou Q*, Li F, Yu Y, Yin X*, Wang J*. Genetic Incorporation of N(ε)-Formyllysine, a New Histone Post-translational Modification. Chembiochem. 2015, 16(10):1440-1442. DOI: 10.1002/cbic.201500170.

51. Zheng Y, Yu F, Wu Y, Si L, Xu H, Zhang C, Xia Q, Xiao S, Wang Q, He Q, Chen P, Wang J, Taira K, Zhang L, Zhou D*. Broadening the versatility of lentiviral vectors as a tool in nucleic acid research via genetic code expansion. Nucleic Acids Res. 2015, 43(11): e73. DOI: 10.1093/nar/gkv202.

52. Li F, Dong J, Hu X, Gong W, Li J, Shen J, Tian H, Wang J*. A covalent approach for site-specific RNA labeling in Mammalian cells. Angew Chem Int Ed Engl. 2015, 54(15):4597-4602. DOI: 10.1002/anie.201410433.

53. Yu Y, Lv X, Li J, Zhou Q, Cui C, Hosseinzadeh P, Mukherjee A, Nilges MJ, Wang J*, Lu Y*. Defining the role of tyrosine and rational tuning of oxidase activity by genetic incorporation of unnatural tyrosine analogs. J Am Chem Soc. 2015 Apr 15;137(14):4594-4597. DOI: 10.1021/ja5109936.

54. Liu X, Jiang L, Li J, Wang L, Yu Y, Zhou Q, Lv X, Gong W, Lu Y, Wang J*. Significant expansion of fluorescent protein sensing ability through the genetic incorporation of superior photo-induced electron-transfer quenchers. J Am Chem Soc. 2014, 136(38):13094-13097. DOI: 10.1021/ja505219r.

55. Pan Y, Jin JH, Yu Y*, Wang J*. Significant enhancement of hPrx1 chaperone activity through lysine acetylation. Chembiochem. 2014, 15(12):1773-1776. DOI: 10.1002/cbic.201402164.

56. Hu C, Chan SI, Sawyer EB, Yu Y, Wang J*. Metalloprotein design using genetic code expansion. Chem Soc Rev. 2014, 43(18):6498-6510. DOI: 10.1039/c4cs00018h.

57. Wu F, Zhang H, Zhou Q, Wu M, Ballard Z, Tian Y, Wang J*, Niu Z*, and Huang Y. Expanding the genetic code for site-specific labelling of tobacco mosaic virus coat protein and building biotin-functionalized virus-like particles. Chem. Commun. 2014, 50, 4007-4009. DOI: 10.1039/c3cc49137d .

58. Bi K, Zheng Y, Gao F, Dong J, Wang J, Wang Y*, Gong W*. Crystal structure of E. coli arginyl-tRNA synthetase and ligand binding studies revealed key residues in arginine recognition. Protein Cell. 2014, 5(2):151-159. DOI: 10.1007/s13238-013-0012-1.

59. Lin Y*, Wang J* & Lu Y*. Functional tuning and expanding of myoglobin by rational protein design. Science China Chemistry. 2014, 57(3): 346-355. DOI: 10.1007/s11426-014-5063-5

60. Zheng Y, Lv X, Wang J*. A genetically encoded sulfotyrosine for VHR function research. Protein Cell. 2013, 4(10):731-734. DOI: 10.1007/s13238-013-3907-y.

61. Lin Y, Wang J*. Structure and function of heme proteins in non-native states: a mini-review. J Inorg Biochem. 2013, 129:162-171. DOI: 10.1016/j.jinorgbio.2013.07.023.

62. Pan C, Liu H, Gong Z, Yu X, Hou X, Xie D, Zhu X, Li H, Tang J, Xu Y, Yu J, Zhang L, Fang H, Xiao K, Chen Y, Wang J, Pang Q, Chen W*, Sun J*. Cadmium is a potent inhibitor of PPM phosphatases and targets the M1 binding site. Sci Rep. 2013, 3: 2333. DOI: 10.1038/srep02333.

63. Li F, Zhang H, Sun Y, Pan Y, Zhou J, Wang J*. Expanding the genetic code for photoclick chemistry in E. coli, mammalian cells, and A. thaliana. Angew Chem Int Ed Engl. 2013, 52(37):9700-9704. DOI: 10.1002/anie.201303477.

64. Lin Y, Sawyer E*, Wang J*. Rational heme protein design: all roads lead to Rome. Chem Asian J. 2013, 8(11):2534-2544. DOI: 10.1002/asia.201300291.

65. Liu X, Li J, Hu C, Zhou Q, Zhang W, Hu M, Zhou J, Wang J*. Significant expansion of the fluorescent protein chromophore through the genetic incorporation of a metal-chelating unnatural amino acid. Angew Chem Int Ed Engl. 2013, 52(18):4805-4809. DOI: 10.1002/anie.201301307.

66. Li F, Shi P, Li J, Yang F, Wang T, Zhang W, Gao F, Ding W, Li D, Li J, Xiong Y, Sun J, Gong W*, Tian C, Wang J*. A genetically encoded 19F NMR probe for tyrosine phosphorylation. Angew Chem Int Ed Engl. 2013, 52(14):3958-3962. DOI: 10.1002/anie.201300463.

67. Xu X, Hu X*, Wang J*. A new synthetic protocol for coumarin amino acid. Beilstein J Org Chem. 2013, 9:254-259. DOI: 10.3762/bjoc.9.30.

68. Zhou Q, Hu M, Zhang W, Jiang L, Perrett S, Zhou J, Wang J*. Probing the function of the Tyr-Cys cross-link in metalloenzymes by the genetic incorporation of 3-methylthiotyrosine. Angew Chem Int Ed Engl. 2013, 52(4):1203-1207. DOI: 10.1002/anie.201207229.

69. Yu Z, Pan Y, Wang Z, Wang J*, Lin Q*. Genetically encoded cyclopropene directs rapid, photoclick-chemistry-mediated protein labeling in mammalian cells. Angew Chem Int Ed Engl. 2012, 51(42):10600-10604. DOI: 10.1002/anie.201205352.

70. Liu X, Li J, Dong J, Hu C, Gong W*, Wang J*. Genetic incorporation of a metal-chelating amino acid as a probe for protein electron transfer. Angew Chem Int Ed Engl. 2012, 51(41):10261-10265. DOI: 10.1002/anie.201204962.

71. Hua T, Wu D, Ding W, Wang J, Shaw N*, Liu Z*. Studies of human 2,4-dienoyl CoA reductase shed new light on peroxisomal β-oxidation of unsaturated fatty acids. J Biol Chem. 2012, 287(34):28956-28965. DOI: 10.1074/jbc.M112.385351.

72. Liu X, Yu Y, Hu C, Zhang W, Lu Y*, Wang J*. Significant increase of oxidase activity through the genetic incorporation of a tyrosine-histidine cross-link in a myoglobin model of heme-copper oxidase. Angew Chem Int Ed Engl. 2012, 51(18):4312-4316. DOI: 10.1002/anie.201108756.

73. Charbon G, Wang J, Brustad E, Schultz PG, Horwich AL, Jacobs-Wagner C, Chapman E*. Localization of GroEL determined by in vivo incorporation of a fluorescent amino acid. Bioorg Med Chem Lett. 2011, 21(20):6067-6070. DOI: 10.1016/j.bmcl.2011.08.057.

74. Charbon G, Brustad E, Scott KA, Wang J, Lobner-Olesen A, Schultz PG, Jacobs-Wagner C, Chapman E*. Subcellular protein localization by using a genetically encoded fluorescent amino acid. Chembiochem. 2011, 12(12):1818-1821. DOI: 10.1002/cbic.201100282.

75.Wang J*, Zhang W, Song W, Wang Y, Yu Z, Li J, Wu M, Wang L, Zang J, Lin Q*. A biosynthetic route to photoclick chemistry on proteins. J Am Chem Soc. 2010, 132 (42): 14812 -14818. DOI: 10.1021/ja104350y.

76. Mills JH, Lee HS, Liu CC, Wang J, Schultz PG.*. A genetically encoded direct sensor of antibody-antigen interactions. Chembiochem. 2009, 10(13):2162-2164. DOI: 10.1002/cbic.200900254.

77. Peters FB, Brock A, Wang J, Schultz PG*. Photocleavage of the polypeptide backbone by 2-nitrophenylalanine. Chem Biol. 2009, 16(2):148-152. DOI: 10.1016/j.chembiol.2009.01.013.

78. Guo J, Wang J, Lee JS, Schultz PG.*. Site-specific incorporation of methyl- and acetyl-lysine analogues into recombinant proteins. Angew Chem Int Ed Engl. 2008, 47(34):6399-6401. DOI: 10.1002/anie.200802336.

79. Guo J, Wang J, Anderson JC, Schultz PG*. Addition of an alpha-hydroxy acid to the genetic code of bacteria. Angew Chem Int Ed Engl. 2008, 47(4):722-725. DOI: 10.1002/anie.200704074

80. Wang J, Rosenblatt MM, Suslick KS*. NMR structures of peptide--RuII(porphyrin) complexes. J Am Chem Soc. 2007, 129(46):14124-14125. DOI: 10.1021/ja075532v

81. Wang J, Schiller SM, Schultz PG*. A biosynthetic route to dehydroalanine-containing proteins.  Angew Chem Int Ed Engl. 2007, 46(36):6849-51. DOI: 10.1002/anie.200702305

82. Wang J, Xie J, Schultz PG*. A genetically encoded fluorescent amino acid. J Am Chem Soc. 2006, 128(27):8738-8739. DOI: 10.1021/ja062666k

83. Rosenblatt MM, Wang J, Suslick KS*. De novo designed cyclic-peptide heme complexes. Proc Natl Acad Sci U S A. 2003, 100(23):13140-13145. DOI: 10.1073/pnas.2231273100

84. Wang J, Luthey-Schulten ZA, Suslick KS*. Is the olfactory receptor a metalloprotein? Proc Natl Acad Sci U S A. 2003, 100(6):3035-3039. DOI: 10.1073/pnas.262792899

（资料来源：王江云研究员，2024-08-20）