ACS Nano 2016 Feb 24. DOI: 10.1021/acsnano.5b06852
Construction of Nano-Droplet/Adiposome and Artificial Lipid Droplets.
Wang Y, Zhou XM, Ma X, Du Y, Zheng L, Liu P.
Abstract
The lipid droplet (LD) is a cellular organelle that consists of a neutral lipid core with a monolayer-phospholipid membrane and associated proteins. Recent LD studies demonstrate its importance in metabolic diseases and biofuel development. However, the mechanisms governing its formation and dynamics remain elusive. Therefore, we developed an in vitro system to facilitate the elucidation of these mechanisms. We generated sphere-shaped structures with a neutral lipid core and a monolayer-phospholipid membrane by mechanically mixing neutral lipids and phospholipids followed by a two-step purification. We named the nano-droplet "adiposome". We then recruited LD structure-like/resident proteins to the adiposome, including the bacterial MLDS, C. elegans MDT-28/PLIN-1, or mammalian perilipin-2. In addition, adipose triglyceride lipase (ATGL) and apolipoprotein A1 (apo A-I) were recruited to adiposome. We termed the functional protein-coated adiposomes, Artificial Lipid Droplets (ALDs). Using this experimental system, different proteins can be recruited to build ALDs for some biological goals and potential usage in drug delivery.
相关报道：http://www.ibp.cas.cn/kyjz/zxdt/201602/t20160229_4538861.html
文章链接：http://pubs.acs.org/doi/abs/10.1021/acsnano.5b06852
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