Genes & Development，Published in Advance May 5, 2015, doi: 10.1101/gad.259432.115
Structural transitions of centromeric chromatin regulate the cell cycle-dependent recruitment of CENP-N
Junnan Fang1,2, Yuting Liu1,2, Yun Wei1, Wenqiang Deng1,2, Zhouliang Yu1,2, Li Huang1, Yan Teng1, Ting Yao1, Qinglong You1,2, Haihe Ruan1, Ping Chen1, Rui-Ming Xu1 and Guohong Li1
1 National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China;
2 University of Chinese Academy of Sciences, Beijing 100049, China
Corresponding author: liguohong@sun5.ibp.ac.cn
Abstract
Specific recognition of centromere-specific histone variant CENP-A-containing chromatin by CENP-N is an essential process in the assembly of the kinetochore complex at centromeres prior to mammalian cell division. However, the mechanisms of CENP-N recruitment to centromeres/kinetochores remain unknown. Here, we show that a CENP-A-specific RG loop (Arg80/Gly81) plays an essential and dual regulatory role in this process. The RG loop assists the formation of a compact “ladder-like” structure of CENP-A chromatin, concealing the loop and thus impairing its role in recruiting CENP-N. Upon G1/S-phase transition, however, centromeric chromatin switches from the compact to an open state, enabling the now exposed RG loop to recruit CENP-N prior to cell division. Our results provide the first insights into the mechanisms by which the recruitment of CENP-N is regulated by the structural transitions between compaction and relaxation of centromeric chromatin during the cell cycle.
相关报道：
http://www.ibp.cas.cn/kyjz/zxdt/201505/t20150506_4350632.html
http://www.cas.cn/syky/201505/t20150506_4351062.shtml
文章链接：http://genesdev.cshlp.org/content/early/2015/05/04/gad.259432.115
附件下载：