Significant Expansion of the Fluorescent Protein Chromophore through the Genetic Incorporation of a ......Acid，Angewandte Chemie International Edition，online: 28 MAR 2013

Angewandte Chemie International Edition，Article first published online: 28 MAR 2013，DOI: 10.1002/anie.201301307

Significant Expansion of the Fluorescent Protein Chromophore through the Genetic Incorporation of a Metal-Chelating Unnatural Amino Acid

Xiaohong Liu ^1,？, Jiasong Li ^1,2,？, Cheng Hu1, Qing Zhou^1, Wei Zhang ^1, Meirong Hu^1, Juanzuo Zhou^1, Jiangyun Wang^1,*

Abstract

A novel metal-chelating unnatural amino acid with an 8-hydroxyquinoline group (HqAla) can be enzymatically incorporated into GFP (see scheme). Substituting a Tyr residue in the chromophore of FPs with HqAla results in significantly red-shifted excitation and emission maxima. The crystal structure of superfolder GFP bearing HqAla in its chromophore shows the structural basis for these red shifts.

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