CRISPR screening uncovers nucleolar RPL22 as a heterochromatin destabilizer and senescence driver
Hong-Yu Li, Min Wang, Xiaoyu Jiang, Yaobin Jing, Zeming Wu, Yifang He, Kaowen Yan, Shuhui Sun, Shuai Ma, Zhejun Ji, Si Wang, Juan Carlos Izpisua Belmonte, Jing Qu, Weiqi Zhang, Taotao Wei, Guang-Hui Liu
Abstract
Dysfunction of the ribosome manifests during cellular senescence and contributes to tissue aging, functional decline, and development of aging-related disorders in ways that have remained enigmatic. Here, we conducted a comprehensive CRISPR-based loss-of-function (LOF) screen of ribosome-associated genes (RAGs) in human mesenchymal progenitor cells (hMPCs). Through this approach, we identified ribosomal protein L22 (RPL22) as the foremost RAG whose deficiency mitigates the effects of cellular senescence. Consequently, absence of RPL22 delays hMPCs from becoming senescent, while an excess of RPL22 accelerates the senescence process. Mechanistically, we found in senescent hMPCs, RPL22 accumulates within the nucleolus. This accumulation triggers a cascade of events, including heterochromatin decompaction with concomitant degradation of key heterochromatin proteins, specifically heterochromatin protein 1γ (HP1γ) and heterochromatin protein KRAB-associated protein 1 (KAP1). Subsequently, RPL22-dependent breakdown of heterochromatin stimulates the transcription of ribosomal RNAs (rRNAs), triggering cellular senescence. In summary, our findings unveil a novel role for nucleolar RPL22 as a destabilizer of heterochromatin and a driver of cellular senescence, shedding new light on the intricate mechanisms underlying the aging process.
附件下载：
CRISPR screening uncovers nucleolar RPL22 as a heterochromatin destabilizer and senescence driver, Nucleic Acids Res, 11 Sep 2024
Nucleic Acids Research, 11 September, 2024, DOI：https://doi.org/10.1093/nar/gkae740
CRISPR screening uncovers nucleolar RPL22 as a heterochromatin destabilizer and senescence driver
Hong-Yu Li, Min Wang, Xiaoyu Jiang, Yaobin Jing, Zeming Wu, Yifang He, Kaowen Yan, Shuhui Sun, Shuai Ma, Zhejun Ji, Si Wang, Juan Carlos Izpisua Belmonte, Jing Qu, Weiqi Zhang, Taotao Wei, Guang-Hui Liu
Abstract
Dysfunction of the ribosome manifests during cellular senescence and contributes to tissue aging, functional decline, and development of aging-related disorders in ways that have remained enigmatic. Here, we conducted a comprehensive CRISPR-based loss-of-function (LOF) screen of ribosome-associated genes (RAGs) in human mesenchymal progenitor cells (hMPCs). Through this approach, we identified ribosomal protein L22 (RPL22) as the foremost RAG whose deficiency mitigates the effects of cellular senescence. Consequently, absence of RPL22 delays hMPCs from becoming senescent, while an excess of RPL22 accelerates the senescence process. Mechanistically, we found in senescent hMPCs, RPL22 accumulates within the nucleolus. This accumulation triggers a cascade of events, including heterochromatin decompaction with concomitant degradation of key heterochromatin proteins, specifically heterochromatin protein 1γ (HP1γ) and heterochromatin protein KRAB-associated protein 1 (KAP1). Subsequently, RPL22-dependent breakdown of heterochromatin stimulates the transcription of ribosomal RNAs (rRNAs), triggering cellular senescence. In summary, our findings unveil a novel role for nucleolar RPL22 as a destabilizer of heterochromatin and a driver of cellular senescence, shedding new light on the intricate mechanisms underlying the aging process.
文章链接：https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkae740/7754987
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