Molecular-scale axial localization by repetitive optical selective exposure
Lusheng Gu, Yuanyuan Li, Shuwen Zhang, Maoge Zhou, Yanhong Xue, Weixing Li, Tao Xu & Wei Ji
Abstract
We introduce an axial localization with repetitive optical selective exposure (ROSE-Z) method for super-resolution imaging. By using an asymmetric optical scheme to generate interference fringes, a <2 nm axial localization precision was achieved with only ~3,000 photons, which is an approximately sixfold improvement compared to previous astigmatism methods. Nanoscale three-dimensional and two-color imaging was demonstrated, illustrating how this method achieves superior performance and facilitates the investigation of cellular nanostructures.
附件下载：
Molecular-scale axial localization by repetitive optical selective exposure, Nat Methods, 1 Apr 2021
Nature Methods, 11 April, 2021, DOI：https://dx.doi.org/10.1038/s41592-021-01099-2
Molecular-scale axial localization by repetitive optical selective exposure
Lusheng Gu, Yuanyuan Li, Shuwen Zhang, Maoge Zhou, Yanhong Xue, Weixing Li, Tao Xu & Wei Ji
Abstract
We introduce an axial localization with repetitive optical selective exposure (ROSE-Z) method for super-resolution imaging. By using an asymmetric optical scheme to generate interference fringes, a <2 nm axial localization precision was achieved with only ~3,000 photons, which is an approximately sixfold improvement compared to previous astigmatism methods. Nanoscale three-dimensional and two-color imaging was demonstrated, illustrating how this method achieves superior performance and facilitates the investigation of cellular nanostructures.
文章链接：https://www.nature.com/articles/s41592-021-01099-2
相关报道：http://www.ibp.cas.cn/kyjz/zxdt/202104/t20210401_5988449.html