Structural and dynamic mechanisms for coupled folding and tRNA recognition of a translational T-box riboswitch, Nat Commun, 15 Nov 2023

Nature Communications, 15 November, 2023, DOI：https://doi.org/10.1038/s41467-023-43232-z

Structural and dynamic mechanisms for coupled folding and tRNA recognition of a translational T-box riboswitch

Xiaolin Niu, Zhonghe Xu, Yufan Zhang, Xiaobing Zuo, Chunlai Chen & Xianyang Fang 

Abstract

T-box riboswitches are unique riboregulators where gene regulation is mediated through interactions between two highly structured RNAs. Despite extensive structural insights, how RNA-RNA interactions drive the folding and structural transitions of T-box to achieve functional conformations remains unclear. Here, by combining SAXS, single-molecule FRET and computational modeling, we elaborate the folding energy landscape of a translational T-box aptamer consisting of stems I, II and IIA/B, which Mg²⁺-induced global folding and tRNA binding are cooperatively coupled. smFRET measurements reveal that high Mg²⁺ stabilizes IIA/B and its stacking on II, which drives the pre-docking of I and II into a competent conformation, subsequent tRNA binding promotes docking of I and II to form a high-affinity tRNA binding groove, of which the essentiality of IIA/B and S-turn in II is substantiated with mutational analysis. We highlight a delicate balance among Mg²⁺, the intra- and intermolecular RNA-RNA interactions in modulating RNA folding and function.

文章链接：https://www.nature.com/articles/s41467-023-43232-z

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