Chiral Carbon Dots Mimicking Topoisomerase I To Mediate the Topological Rearrangement of Supercoiled DNA Enantioselectively, Angew Chem Int Edit, 25 Mar 2020

发布时间:2020-03-25

Angewandte Chemie International Edition, 25 March, 2020, DOI:http://dx.doi.org/10.1002/anie.202002904

Chiral Carbon Dots Mimicking Topoisomerase I To Mediate the Topological Rearrangement of Supercoiled DNA Enantioselectively

Prof. Feng Li  Shuai Li  Xiaocui Guo  Yuhang Dong  Dr. Chi Yao  Prof. Yangping Liu  Dr. Yuguang Song Dr. Xiaoli Tan  Prof. Lizeng Gao  Prof. Dayong Yang

Abstract

Nanomaterials with enzyme‐mimetic activities are possible alternatives to natural enzymes. Mimicking enzymatic enantioselectivity remains a great challenge. Herein, we report that cysteine‐derived chiral carbon dots (CDs) can mimic topoisomerase?I to mediate topological rearrangement of supercoiled DNA enantioselectively. d ‐CDs can more effectively catalyze the topological transition of plasmid DNA from supercoiled to nicked open‐circular configuration than l ‐CDs. Experiments suggest the underlying mechanism: d ‐CDs intercalatively bind with DNA double helix more strongly than l ‐CDs; the intercalative CDs can catalyze the production of hydroxyl radicals to cleave phosphate backbone in one strand of the double helix, leading to topological rearrangement of supercoiled DNA. Molecular dynamics (MD) simulation show that the stronger affinity for hydrogen‐bond formation and hydrophobic interaction between d ‐cysteine and DNA than that of l ‐cysteine is the origin of enantioselectivity.

文章链接:https://onlinelibrary.wiley.com/doi/full/10.1002/anie.202002904

 

 


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