中国科学院蛋白质科学研究平台

　　2008年度新技术系列报告之一：

多角度激光光散射技术报告会

会议通知

　　会议时间：2008年4月23日（周三）9：00-11：00

　　会议地点：中国科学院生物物理所大报告厅

　　报告单位：美国怀雅特技术公司（Wyatt Technology Corporation）

　　会议主题：多角度激光光散射和场流分离技术及应用

　　 （内容简介见下页）

　　主持人： 孙飞 博士（生物物理研究所）

　　报告人： 陈泓 博士 （美国怀雅特技术公司）

　　欢迎研究所感兴趣的老师、同学到会！

科学研究平台

2008年4月21日

Light Scattering for Biopolymer Characterization

　　Objective: To provide an introduction on how static and dynamic light scattering detectors determine absolute molar mass, size, and conformation of biopolymers in solution. Various applications and new tools including protein conjugate analysis, field flow fractionation, and automated DLS will be discussed.

　　Speaker: Dr. Michelle Chen, Head of Analytical Services at Wyatt Technology, graduated from the Chemical Engineering Department, Yale University, under the advisory of late Professor Csaba Horvath. At WTC, she has been working with polymer characterization using different light scattering techniques in conjunction with various branches of HPLC and FFF.

　　Agenda:

　　Introduction to light-scattering theory 15 min

　　How absolute molar mass and size are determined

　　Flowing mode – on-line MALS and QELS

　　MALS-UV-RI: measuring extinction coefficient and protein conjugates

　　Batch mode - use of flow cell, scintillation vials and microCuvette

　　Polymer characterization using MALS/QELS detection 10 min

　　Measure absolute MW and hydrodynamic radius

　　Determine oligomerization states of proteins and aggregation

　　Provide preliminary structural analysis

　　Characterize protein conjugates, membrane proteins

　　Obtain size/distribution information of micelles and liposomes

　　Example applications 20 min

　　Questions and comments 15 min