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Crystal structure of human LDB1 in complex with SSBP2, PNAS, 31 Dec 2019
2019-12-31 | 【     】【打印】【关闭

PNAS31 December, 2019,DOI:https://doi.org/10.1073/pnas.1914181117

Crystal structure of human LDB1 in complex with SSBP2

Hongyang Wang, Juhyun Kim, Zhizhi Wang, Xiao-Xue Yan, Ann Dean, and Wenqing Xu

Abstract

The Lim domain binding proteins (LDB1 and LDB2 in human and Chip in Drosophila) play critical roles in cell fate decisions through partnership with multiple Lim-homeobox and Lim-only proteins in diverse developmental systems including cardiogenesis, neurogenesis, and hematopoiesis. In mammalian erythroid cells, LDB1 dimerization supports long-range connections between enhancers and genes involved in erythropoiesis, including the β-globin genes. Single-stranded DNA binding proteins (SSBPs) interact specifically with the LDB/Chip conserved domain (LCCD) of LDB proteins and stabilize LDBs by preventing their proteasomal degradation, thus promoting their functions in gene regulation. The structural basis for LDB1 self-interaction and interface with SSBPs is unclear. Here we report a crystal structure of the human LDB1/SSBP2 complex at 2.8-angstrom resolution. The LDB1 dimerization domain (DD) contains an N-terminal nuclear transport factor 2 (NTF2)-like subdomain and a small helix 4–helix 5 subdomain, which together form the LDB1 dimerization interface. The 2 LCCDs in the symmetric LDB1 dimer flank the core DDs, with each LCCD forming extensive interactions with an SSBP2 dimer. The conserved linker between LDB1 DD and LCCD covers a potential ligand-binding pocket of the LDB1 NTF2-like subdomain and may serve as a regulatory site for LDB1 structure and function. Our structural and biochemical data provide a much-anticipated structural basis for understanding how LDB1 and the LDB1/SSBP interactions form the structural core of diverse complexes mediating cell choice decisions and long-range enhancer–promoter interactions.

文章链接:https://www.pnas.org/content/early/2019/12/30/1914181117

相关报道:http://www.ibp.cas.cn/kyjz/zxdt/202001/t20200106_5482645.html

 

 

 

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