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S‐Click Reaction for Isotropic Orientation of Oxidases on Electrodes to Promote Electron Transfer at Low Potentials, Angew Chem Int Edit, 04 Oct 2019

发布时间:2019年10月04日

Angewandte Chemie International Edition04 October, 2019,DOI:https://doi.org/10.1002/anie.201909203

S‐Click Reaction for Isotropic Orientation of Oxidases on Electrodes to Promote Electron Transfer at Low Potentials

Dr. Lin Xia  Dr. Ming‐Jie Han  Lu Zhou  Dr. Aiping Huang  Zhaoya Yang  Tianyuan Wang  Dr. Fahui Li  Dr. Lu Yu  Prof. Changlin Tian  Zhongsheng Zang  Prof. Qing‐Zheng Yang  Prof. Chenli Liu  Dr. Wenxu Hong Prof. Yi Lu  Prof. Lital Alfonta  Prof. Jiangyun Wan

Abstract

Electrochemical sensors are essential for point‐of‐care testing (POCT) and wearable sensing devices. Establishing an efficient electron transfer route between redox enzymes and electrodes is key for converting enzyme‐catalyzed reactions into electrochemical signals, and for the development of robust, sensitive, and selective biosensors. We demonstrate that the site‐specific incorporation of a novel synthetic amino acid (2‐amino‐3‐(4‐mercaptophenyl)propanoic acid) into redox enzymes, followed by an S‐click reaction to wire the enzyme to the electrode, facilitates electron transfer. The fabricated biosensor demonstrated real‐time and selective monitoring of tryptophan (Trp) in blood and sweat samples, with a linear range of 0.02–0.8?mm. Further developments along this route may result in dramatic expansion of portable electrochemical sensors for diverse health‐determination molecules.

文章链接:https://onlinelibrary.wiley.com/doi/full/10.1002/anie.201909203

相关报道:http://www.ibp.cas.cn/kyjz/zxdt/201910/t20191008_5404039.html

 

 

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