最新重要论文(影响因子PNAS及以上)

A Pandas complex adapted for piRNA-guided transcriptional silencing and heterochromatin formation, Nat Cell Biol, 30 Sep 2019

发布时间:2019年09月30日

Nature Cell Biology30 September, 2019,DOI:https://doi.org/10.1038/s41556-019-0396-0

A Pandas complex adapted for piRNA-guided transcriptional silencing and heterochromatin formation

Kang Zhao, Sha Cheng, Na Miao, Ping Xu, Xiaohua Lu, Yuhan Zhang, Ming Wang, Xuan Ouyang, Xun Yuan, Weiwei Liu, Xin Lu, Peng Zhou, Jiaqi Gu, Yiqun Zhang, Ding Qiu, Zhaohui Jin, Chen Su, Chao Peng, Jian-Hua Wang, Meng-Qiu Dong, Youzhong Wan, Jinbiao Ma, Hong Cheng, Ying Huang & Yang Yu

Abstract

The repression of transposons by the Piwi-interacting RNA (piRNA) pathway is essential to protect animal germ cells. In Drosophila, Panoramix enforces transcriptional silencing by binding to the target-engaged Piwi–piRNA complex, although the precise mechanisms by which this occurs remain elusive. Here, we show that Panoramix functions together with a germline-specific paralogue of a nuclear export factor, dNxf2, and its cofactor dNxt1 (p15), to suppress transposon expression. The transposon RNA-binding protein dNxf2 is required for animal fertility and Panoramix-mediated silencing. Transient tethering of dNxf2 to nascent transcripts leads to their nuclear retention. The NTF2 domain of dNxf2 competes dNxf1 (TAP) off nucleoporins, a process required for proper RNA export. Thus, dNxf2 functions in a Panoramix–dNxf2-dependent TAP/p15 silencing (Pandas) complex that counteracts the canonical RNA exporting machinery and restricts transposons to the nuclear peripheries. Our findings may have broader implications for understanding how RNA metabolism modulates heterochromatin formation.

文章链接:https://www.nature.com/articles/s41556-019-0396-0

相关报道:http://www.ibp.cas.cn/kyjz/zxdt/201909/t20190929_5402610.html

 

 

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