最新重要论文(影响因子PNAS及以上)

The late stage of COPI vesicle fission requires shorter forms of phosphatidic acid and diacylglycerol,Nature Comm, 30 Jul 2019

发布时间:2019年07月30日

Nature Communications30 July, 2019,DOI:http://dx.doi.org/10.1038/s41467-019-11324-4

The late stage of COPI vesicle fission requires shorter forms of phosphatidic acid and diacylglycerol

Seung-Yeol Park, Jia-Shu Yang, Zhen Li, Pan Deng, Xiaohong Zhu, David Young, Maria Ericsson, Ruben L. H. Andringa, Adriaan J. Minnaard, Chunmei Zhu, Fei Sun, D. Branch Moody, Andrew J. Morris, Jun Fan & Victor W. Hsu

Abstract

Studies on vesicle formation by the Coat Protein I (COPI) complex have contributed to a basic understanding of how vesicular transport is initiated. Phosphatidic acid (PA) and diacylglycerol (DAG) have been found previously to be required for the fission stage of COPI vesicle formation. Here, we find that PA with varying lipid geometry can all promote early fission, but only PA with shortened acyl chains promotes late fission. Moreover, diacylglycerol (DAG) acts after PA in late fission, with this role of DAG also requiring shorter acyl chains. Further highlighting the importance of the short-chain lipid geometry for late fission, we find that shorter forms of PA and DAG promote the vesiculation ability of COPI fission factors. These findings advance a general understanding of how lipid geometry contributes to membrane deformation for vesicle fission, and also how proteins and lipids coordinate their actions in driving this process.

文章链接:https://www.nature.com/articles/s41467-019-11324-4

 

 

  附件下载: