网站地图 联系我们搜索内部网English | 中国科学院
首页 所况简介 机构设置 科研队伍 科学研究平台 院地合作 党群园地 国际交流 博士后 研究生教育 科学传播 信息公开
科研进展
成果报道
最新重要论文(影响因子PNAS及以上)
发表论文数据库
所级学术报告
科学成果
发表论文
专著
专利
获奖
专题
所史丛书
所庆专辑
建所50周年画册
现在位置:首页 > 科研进展 > 最新重要论文(影响因子PNAS及以上)
RNA Targets Ribogenesis Factor WDR43 to Chromatin for Transcription and Pluripotency Control,Mol Cell, 11 Jul 2019
2019-07-11 | 【     】【打印】【关闭

Molecular Cell11 July, 2019,DOI:https://doi.org/10.1016/j.molcel.2019.05.007

RNA Targets Ribogenesis Factor WDR43 to Chromatin for Transcription and Pluripotency Control

Xianju Bi, Yanhui Xu, Tong Li, Xinmin Li, Wenzhi Li, Wen Shao, Kaili Wang, Ge Zhan, Zhongyang Wu, Wei Liu, J. Yuyang Lu, Liang Wang, Jicheng Zhao, Jiawei Wu, Jie Na, Guohong Li, Pilong Li, Xiaohua Shen

Summary

Transcription regulation underlies stem cell function and development. Here, we elucidate an unexpected role of an essential ribogenesis factor, WDR43, as a chromatin-associated RNA-binding protein (RBP) and release factor in modulating the polymerase (Pol) II activity for pluripotency regulation. WDR43 binds prominently to promoter-associated noncoding/nascent RNAs, occupies thousands of gene promoters and enhancers, and interacts with the Pol II machinery in embryonic stem cells (ESCs). Nascent transcripts and transcription recruit WDR43 to active promoters, where WDR43 facilitates releases of the elongation factor P-TEFb and paused Pol II. Knockdown of WDR43 causes genome-wide defects in Pol II release and pluripotency-associated gene expression. Importantly, auxin-mediated rapid degradation of WDR43 drastically reduces Pol II activity, precluding indirect consequences. These results reveal an RNA-mediated recruitment and feedforward regulation on transcription and demonstrate an unforeseen role of an RBP in promoting Pol II elongation and coordinating high-level transcription and translation in ESC pluripotency.

文章链接:https://www.sciencedirect.com/science/article/pii/S1097276519303600?via%3Dihub

 

 

评 论
          
版权所有:中国科学院生物物理研究所     京ICP备05002792号 京公网安备 110402500011 号
地址:北京市朝阳区大屯路15号(100101) 电话:010-64889872
意见反馈联系人:侯文茹 电子邮件:houwenru@ibp.ac.cn