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现在位置:首页 > 科研进展 > 最新重要论文(影响因子PNAS及以上)
Diverse Mechanisms of CRISPR-Cas9 Inhibition by Type IIC Anti-CRISPR Proteins, Mol Cell, 18 Apr 2019
2019-04-18 | 【     】【打印】【关闭

Molecular Cell, 18 April, 2019, DOI: http://dx.doi.org/10.1016/j.molcel.2019.01.038

Diverse Mechanisms of CRISPR-Cas9 Inhibition by Type IIC Anti-CRISPR Proteins

Yalan Zhu, Ang Gao, Qi Zhan, Yong Wang, Han Feng, Songqing Liu, Guangxia Gao, Alexander Serganov, Pu Gao

Abstract

Anti-CRISPR proteins (Acrs) targeting CRISPR-Cas9 systems represent natural “off switches” for Cas9-based applications. Recently, AcrIIC1, AcrIIC2, and AcrIIC3 proteins were found to inhibit Neisseria meningitidis Cas9 (NmeCas9) activity in bacterial and human cells. Here we report biochemical and structural data that suggest molecular mechanisms of AcrIIC2- and AcrIIC3-mediated Cas9 inhibition. AcrIIC2 dimer interacts with the bridge helix of Cas9, interferes with RNA binding, and prevents DNA loading into Cas9. AcrIIC3 blocks the DNA loading step through binding to a non-conserved surface of the HNH domain of Cas9. AcrIIC3 also forms additional interactions with the REC lobe of Cas9 and induces the dimerization of the AcrIIC3-Cas9 complex. While AcrIIC2 targets Cas9 orthologs from different subtypes, albeit with different efficiency, AcrIIC3 specifically inhibits NmeCas9. Structure-guided changes in NmeCas9 orthologs convert them into anti-CRISPR-sensitive proteins. Our studies provide insights into anti-CRISPR-mediated suppression mechanisms and guidelines for designing regulatory tools in Cas9-based applications.

文章链接:https://www.sciencedirect.com/science/article/pii/S1097276519300589?via%3Dihub#!

 

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