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Standardized assays for determining the catalytic activity and kinetics of peroxidase-like nanozymes, Nat Protoc, 2 July 2018
2018-06-05 | 【     】【打印】【关闭

Nat Protoc, 2 July 2018,DOI: 10.1038/s41596-018-0001-1

Standardized assays for determining the catalytic activity and kinetics of peroxidase-like nanozymes

Bing Jiang, Demin Duan, Lizeng Gao, Mengjie Zhou, Kelong Fan, Yan Tang, Juqun Xi, Yuhai Bi, Zhou Tong, George Fu Gao, Ni Xie, Aifa Tang, Guohui Nie, Minmin Liang and Xiyun Yan

Abstract

Nanozymes are nanomaterials exhibiting intrinsic enzyme-like characteristics that have increasingly attracted attention,owing to their high catalytic activity, low cost and high stability. This combination of properties has enabled a broad spectrum of applications, ranging from biological detection assays to disease diagnosis and biomedicine development.Since the intrinsic peroxidase activity of Fe3O4 nanoparticles (NPs) was first reported in 2007, >40 types of nanozymes have been reported that possess peroxidase-, oxidase-, haloperoxidase- or superoxide dismutase–like catalytic ativities.Given the complex interdependence of the physicochemical properties and catalytic characteristics of nanozymes, it is important to establish a standard by which the catalytic activities and kinetics of various nanozymes can be quantitatively compared and that will benefit the development of nanozyme-based detection and diagnostic technologies. Here, we first present a protocol for measuring and defining the catalytic activity units and kinetics for peroxidase nanozymes, the most widely used type of nanozyme. In addition, we describe the detailed experimental procedures for a typical nanozyme strip–based biological detection test and demonstrate that nanozyme-based detection is repeatable and reliable when guided by the presented nanozyme catalytic standard. The catalytic activity and kinetics assays for a nanozyme can be performed within 4 h.

文章链接:https://www.nature.com/articles/s41596-018-0001-1

相关报道:http://www.ibp.cas.cn/kyjz/zxdt/201807/t20180703_5036928.html

 

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