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现在位置:首页 > 科研进展 > 最新重要论文(影响因子PNAS及以上)
Highly photostable, reversibly photoswitchable fluorescent protein... for live-cell superresolution microscopy, PNAS 2016; published ahead of print August 25, 2016
2016-09-01 | 【     】【打印】【关闭

PNAS 2016 ; published ahead of print August 25, 2016, doi:10.1073/pnas.1611038113

Highly photostable, reversibly photoswitchable fluorescent protein with high contrast ratio for live-cell superresolution microscopy

Xi Zhang, Mingshu Zhang, Dong Li, Wenting He, Jianxin Peng, Eric Betzig, and Pingyong Xu

Abstact

Two long-standing problems for superresolution (SR) fluorescence microscopy are high illumination intensity and long acquisition time, which significantly hamper its application for live-cell imaging. Reversibly photoswitchable fluorescent proteins (RSFPs) have made it possible to dramatically lower the illumination intensities in saturated depletion-based SR techniques, such as saturated depletion nonlinear structured illumination microscopy (NL-SIM) and reversible saturable optical fluorescence transition microscopy. The characteristics of RSFPs most critical for SR live-cell imaging include, first, the integrated fluorescence signal across each switching cycle, which
depends upon the absorption cross-section, effective quantum yield, and characteristic switching time from the fluorescent “on” to “off” state; second, the fluorescence contrast ratio of on/off states; and third, the photostability under excitation and depletion. Up to now, the RSFPs of the Dronpa and rsEGFP (reversibly switchable EGFP) families have been exploited for SR imaging. However, their limited number of switching cycles, relatively low fluorescence signal, and poor contrast ratio under physiological conditions ultimately restrict their utility in time-lapse live-cell imaging and their ability to reach the desired resolution at a reasonable signal-to-noise ratio. Here, we present a truly monomeric RSFP, Skylan-NS, whose properties are optimized for the recently developed patterned activation NL-SIM, which enables low-intensity (∼100 W/cm2) live-cell SR imaging at
∼60-nm resolution at subsecond acquisition times for tens of time points over broad field of view.

相关报道:http://www.ibp.cas.cn/kyjz/zxdt/201608/t20160826_4655539.html

文章链接:http://www.pnas.org/content/early/recent

          http://www.pnas.org/content/early/2016/08/24/1611038113.full.pdf

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