生物大分子重点实验室

　　2013年5月至今，在中科院生物物理所生物大分子国家重点实验室担任PI研究员。2021年3月开始在中科院深圳理工大学（筹）合成生物学院担任名誉院长、讲席教授。

　　1982年毕业于湖北大学生物系，1985年在中科院武汉病毒所获硕士学位（微生物学专业），1995年在中科院微生物所获博士学位（生物化学专业）。

　　1993年在中科院武汉病毒所晋升研究员。1992年起历任中科院武汉病毒所、中科院武汉分院和国家科学技术部基础研究司领导职务。曾先后在加拿大、美国和英国相关学术机构从事合作研究。

　　主要学术兼职：中国生物工程学会副理事长、合成生物学分会主任、生物传感/生物芯片/纳米生物学分会荣誉主任，美国医学与生物工程院 Fellow，英国皇家化学会 Fellow，, 亚洲生物技术协会（AFOB）纳米生物技术/生物传感/生物芯片分会共同创始会长，世界生物传感学术大会常设组委会委员； Biosensors & Bioelectronics、Science China-Life Science、mLife、BioDesign Research、《科学通报》、《中科院院刊》、《合成生物学》、《生物工程学报》、《高等学校化学学报》等学术期刊编委或顾问。国家合成生物学重点专项指南编制专家组组长，科技部生物技术中心专家委员会成员；曾担任中国微生物学会副理事长、中国生物物理学会副理事长和伦敦纳米中心顾问小组成员。

　　曾获中科院首届青年科学家奖(1989)、中科院有突出贡献中青年专家（1994）、湖北省自然科学一等奖（2015）等荣誉。2015年获加拿大Alberta大学荣誉科学博士学位。

　　发表科学论文300余篇（其中SCI收录260余篇），生物传感器和生物芯片相关专著3部。

　　基于生物大分子工程（合成生物学和纳米生物学）发展先进的分析生物技术和生物物理方法，主要包括：

　　◆ 合成细胞传感器与环境和毒物评价

　　◆ 病毒-宿主相互作用可视化动态示踪

　　◆ 高内涵肿瘤细胞抗原谱的构建

发表科学论文300余篇（其中SCI收录260余篇），生物传感器和生物芯片相关专著3部。

一、通讯作者论文（近五年发表）

1. Minhai Chen, Chuang Yan, Yingxin Ma, Xian-En Zhang* (2021). A tandem near-infrared fluorescence complementation system with enhanced fluorescence for imaging protein-protein interactions in vivo. Biomaterials, 268, DOI: 10.1016/j.biomaterials.2020.120544

2. Dianbing Wang*, Mengmeng Cui, Min Li, Xian-En Zhang* (2021). Biosensors for the Detection of Bacillus anthracis. Accounts of chemical research, DOI: 10.1021/acs.accounts.1c00407

3. YingXin Ma, Guobin Mao, Guoqiang Wu, Zongqiang Cui*, Xian-En Zhang*, Weiren Huang* (2021). CRISPR-dCas9-Guided and Telomerase-Responsive Nanosystem for Precise Anti-Cancer Drug Delivery. ACS Applied Materials & Interfaces, 13(7), 7890-7896.

4. Yingxin Ma, Guobin Mao, Guoqiang Wu, Minghai Chen, Fujun Qin, Luping Zheng, Xian-En Zhang* (2021).Dual-Fluorescence Labeling Pseudovirus for Real-Time Imaging of Single SARS-CoV-2 Entry in Respiratory Epithelial Cells. ACS Applied Materials & Interfaces, DOI:10.1021/acsami.1c03897.

5. Luping Zheng, Yingxin Ma, Minghai Chen, Guoqiang Wu, Chuang Yan, Xian-En Zhang* (2021). SARS-CoV-2 spike protein receptor-binding domain N-glycans facilitate viral internalization in respiratory epithelial cells. Biochemical and Biophysical Research Communications, 579,69-75.

6. Yang Zheng, Qing Zhang, Ashaq Ali, Ke Li, Nan Shao, Xiaoli Zhou, Zhiqin Ye, Xiaomin Chen, Shanshan Cao, Jing Cui, Juan Zhou, Dianbing Wang, Baidong Hou, Min Li, Mengmeng Cui, Lihua Deng, Xinyi Sun, Qian Zhang, Qinfang Yang, Yong Li, Hui Wang, Yake Lei, Bo Yu, Yegang Cheng*, Xiaolin Tong*, Dong Men*, Xian-En Zhang* (2021). Sustainability of SARS-CoV-2 Induced Humoral Immune Responses in COVID-19 Patients from Hospitalization to Convalescence Over Six Months. Virologica Sinica, 36(5), 869-878.

7. Chakavak Esmaeili, Jin Song, Yuliang Li, Lanqun Mao, Huibiao Liu, Fei Wu, Yanhuan Chen, Chenli Liu, Xian-En Zhang* (2021). Synthesis and Application of Graphdiyne Oxide-Polyurethane Nanocomposite Yield a Highly Sensitive Non-Enzyme Glucose Sensor. Journal of the Electrochemical Society, 168(7), 077520.

8. Yuwei Zhang, Juan Zhou, Yuchen Zhang, Tiangang Liu, Xiaoyun Lu, Dong Men*, Xian-En Zhang* (2021). Auxiliary Module Promotes the Synthesis of Carboxysomes in E. coli to Achieve High-Efficiency CO2 Assimilation. ACS Synthetic Biology, 10(4), 707-715.

9. Minghai Chen, Xian-En Zhang* (2021). Construction and applications of SARS-CoV-2 pseudoviruses: a mini review. International Journal of Biological Sciences, 17(6),1574-1580.

10. Minghai Chen*, Chuang Yan, Fujun Qin, Luping Zheng, Xian-En Zhang* (2021). The intraviral protein-protein interaction of SARS-CoV-2 reveals the key role of N protein in virus-like particle assembly. International Journal of Biological Sciences, 17(14), 3889-3897.

11. Rui Gong, Dianbing Wang, Abbas Ghulam, Shimin Li, Qian Liu, Mengmeng Cui, Xian-En Zhang* (2021). A switch-on molecular biosensor for detection of caspase-3 and imaging of apoptosis of cells. Science China-Life Sciences, DOI:10.1007/s11427-021-1986-7.

12. Jianlin Zhang, Shimin Li, Ni Xie, Guohui Nie, Aifa Tang*, Xian-En Zhang*, Minmin Liang*, Xiyun Yan* (2021). A natural nanozyme in life is found: the iron core within ferritin shows superoxide dismutase catalytic activity. Science China-Life Sciences, DOI: 10.1007/s11427-020-1865-2.

13. Xiaohua Chen, Juan Zhou, Chen Chen, Baidong Hou, Ashaq Ali, Feng Li, Zhaolin Hua, Yingtao Wu, Qin Yang, Min Chen, Rong Zhang, Qianchuan Huang*, Jinya Ding*, Xian-En Zhang*, Dong Men* (2021). Consecutive Monitoring of Interleukin-6 Is Needed for COVID-19 Patients. Virologica Sinica, 36(5), 1093-1096.

14. Xiaoli Zhou, Yana Zhou, Ashaq Ali, Cuiqin Liang, Zhiqin Ye, Xiaomin Chen, Qing Zhang, Lihua Deng, Xinyi Sun, Qian Zhang, Jihong Luo, Wei Li, Kun Zhou, Shanshan Cao, Xiaowei Zhang, Xiaodong Li*, Xian-En Zhang*, Zongqiang Cui*, Dong Men* (2021). Case Report: A Re-Positive Case of SARS-CoV-2 Associated With Glaucoma. Frontiers in Immunology, 12, 701295.

15. Netrapal Singh, Lin Huang, Dian-Bing Wang, Nan Shao, Xian-En Zhang* (2020). Simultaneous Detection of a Cluster of Differentiation Markers on Leukemia-Derived Exosomes by Multiplex Immuno-Polymerase Chain Reaction via Capillary Electrophoresis Analysis. Analytical Chemistry, 92(15):10569-10577.

16. Xia Li, Dianbing Wang, Zongqiang Cui, Qin Li, Min Li, Yinxin Ma, Qinxue Hu, Yikai Zhou*, Xian-En Zhang* (2020). HIV-1 viral cores enter the nucleus collectively through the nuclear endocytosis-like pathway. Science China-Life Sciences, DOI: 10.1007/s11427-020-1716-x.

17. Jin Song, Yiwei Li, Fang Yin, Zhitao Zhang, Dingkun Ke, Dian-bing Wang, Qipeng Yuan*, Xian-En Zhang* (2020). Enhanced Electrochemical Impedance Spectroscopy Analysis of 3Microbial Biofilms on an Electrochemically In Situ Generated Graphene Interface. ACS sensors, 5(6) 1795-1803.

18. Song Lu, Dian-Bing Wang, Yu Hou, Dongge Guo, Yulin Deng*, Xian-En Zhang* (2020). Illuminating single genomic loci in live cells by reducing nuclear background fluorescence. Science China-Life Sciences, doi: 10.1007/s11427-020-1794-2.

19. Chenchen Xu, Weiwei Zhu, Hanjin Mao, Wenjing Zhang, Gen-Quan Yin, Xian-En Zhang*, Feng Li* (2020). Switch from Polymorphic to Homogenous Self-Assembly of Virus-Like Particles of Simian Virus 40 through Double-Cysteine Substitution. SMALL, DOI: 10.1002/smll.202004484.

20. Wei Feng, Ashley M. Newbigging, Connie Le, Bo Pang, Hanyong Peng, Yiren Cao, Jinjun Wu, Ghulam Abbas, Jin Song, Dian-Bing Wang, Mengmeng Cui, Jeffery Tao, D. Lome, Tyrrell, Xian-En Zhang*, Hongquan Zhang*, X. Chis Le* (2020). Molecular Diagnosis of COVID-19: Challenges and Research Needs. Analytical Chemistry, 15(92),10196-10209.

21. Xian-En Zhang*. Nanobiology-Symphony of bioscience and nanoscience. Science China-Life Sciences, 8(63), 1099-1102.

22. Lin Huang, Ning Gu, Xian-En Zhang*, Dian-Bing Wang* (2019). Light-Inducible Exosome-Based Vehicle for Endogenous RNA Loading and Delivery to Leukemia Cells. Advanced Functional Materials, 29(9):1807189.

23. Yan Xi, Dian-Bing Wang, Tingting Wang, Lin Huang, Xian-En Zhang* (2019). Quantitative Profiling of CD13 on Single Acute Myeloid Leukemia Cells by Super-resolution imaging and its Implication in Targeted Drug Susceptibility Assessment. Nanoscale, 11(4):1737-1744.

24. Dongge Guo, Dian-Bing Wang, Chong Liu, Song Lu, Yu Hou, Xian-En Zhang* (2019). CRISPR-based genomic loci labeling revealed ordered spatial organization of chromatin in living diploid human cells. BBA-Molecular Cell Research, 1866(12):118518.

25. Lingling Li, Chengchen Xu, Wenjing Zhang, Francesco Secundo, Chunyan Li, Zhi-Ping Zhang, Xian-En Zhang*, Feng Li* (2019). Cargo-Compatible Encapsulation in Virus-Based Nanoparticles. Nano Letters, 19(4):2700-2706.

26. Tingting Wang, Guang Li, Dianbing Wang, Feng Li, Dong Men, Tao Hu, Yan Xi, and Xian-En Zhang* (2019). Quantitative profiling of integrin αvβ3 on single cells with quantum dot labeling to reveal the phenotypic heterogeneity of glioblastoma. Nanoscale, 11(39):18224-18231.

27. Yiwei Li, Juan Zhou, Jin Song, Xiaosheng Liang, Zhiping Zhang, Dong Men*, Dianbing Wang, Xian-En Zhang* (2019). Chemical Nature of Electrochemical activation of Carbon Electrodes. Biosensors & Bioelectronics, 144: 111534.

28. Sha Wei, Dianbing Wang, Hua Li, Lijun Bi, Jiaoyu Deng, Guofeng Zhu, Jibin Zhang, Chuanyou Li, Min Li, Yuan Fang, Guimin Zhang, Jian Chen, Shengce Tao, Xian-En Zhang* (2019). Fatty acylCoA synthetase FadD13 regulates proinflammatory cytokine secretion dependent on the NF‐κB signalling pathway by binding to eEF1A1. Cellular Microbiology, e13090.

29. Yuanyuan Chen, Zhengyan Zhan, Hongtai Zhang, Lijun Bi, Xian-En Zhang*, Yu Vincent Fu* (2019). Kinetic analysis of DNA compaction by mycobacterial integration host factor at the single-molecule level. Tuberculosis, 119, 101862.

30. 黄琳，王殿冰，顾宁，张先恩* (2019). 高效装载细胞内源蛋白工程化外泌体的构建. 生物工程学报，(8):1537-1545.

31. Xian-En Zhang* (2018). Forty years of highly synchronous development in China's scientific research and economy. National Science Review, 5 (6): 796-798.

32. Fan Yang, Xiaolei Zuo, Chunhai Fan*, Xian-En Zhang* (2018). Biomacromolecular nanostructures-based interfacial engineering: from precise assembly to precision biosensing. National Science Review, 5 (5): 740-755.

33. Lin Huang, Dian-Bing Wang, Netrapal Singh, Fang Yang, Ning Gu*, Xian-En Zhang* (2018). A dual-signal amplification platform for sensitive fluorescence biosensing of leukemia-derived exosomes. Nanoscale, 10(43): 20289-20295.

34. Ye Yuan, Dian-Bing Wang, Jibin Zhang, Ji Liu, Jian Chen, Xian-En Zhang* (2018). Raman spectra of the GFP-like fluorescent proteins. Biophysics reports, 4(5): 265-272.

35. Jigang Wang, Ti Fang, Ming Li, Wenjing Zhang, Zhi-Ping Zhang, Xian-En Zhang*, Feng Li* (2018). Intracellular delivery of peptide drugs using viral nanoparticles of bacteriophage P22: covalent loading and cleavable release. Journal of Materials Chemistry B, 6(22):3716-3726.

36. Wen Yin, Wei Li, Qin Li, Chong Qin, Zhi-Ping Zhang, Xiaowei Zhang, Xian-En Zhang*, Zongqiang Cui* (2018). Structural Characterization and Analysis of Human Immunodeficiency Virus Encapsulating Quantum Dots. Journal of Nanoscience and Nanotechnology, 18(7):4487-4494.

37. Wenjing Zhang, Xian-En Zhang*, Feng Li* (2018). Virus-Based Nanoparticles of Simian Virus 40 in the Field of Nanobiotechnology. Biotechnology Journal, 13(6).

38. Dong Men, Juan Zhou, Wei Li, Cui-Hua Wei, Yuan-yuan Chen, Kun Zhou, Ying Zheng, Ke Xu, Zhi-Ping Zhang, Xian-En Zhang* (2018). Self-Assembly of Antigen Proteins into Nanowires Greatly Enhances the Binding Affinity for High-Efficiency Target Capture. ACS Appl Mater Interfaces, 10(48):41019-41025.

39. Yingxin Ma, Mingxiu Wang, Wei Li, Zhiping Zhang, Xiaowei Zhang, Guoqiang Wu, Tianwei Tan, Zongqiang Cui*, Xian-En Zhang* (2017). Live Visualization of HIV-1 Proviral DNA Using a Dual-Color-Labeled CRISPR System. Analytical Chemistry, 89(23): 12896-12901.

40. Qin Li, Wei Li, Wen Yin, Jia Guo, Zhi-Ping Zhang, Dejun Zeng, Xiaowei Zhang, Yuntao Wu, Xian-En Zhang*, and Zongqiang Cui* (2017). Single-Particle Tracking of Human Immunodeficiency Virus Type 1 Productive Entry into Human Primary Macrophages. ACS Nano, 11(4):3890-3903.

41. Yingxin Ma, Mingxiu Wang, Wei Li, Zhiping Zhang, Xiaowei Zhang, Tianwei Tan, Xian-En Zhang* & Zongqiang Cui* (2017). Live cell imaging of single genomic loci with quantum dot-labeled TALEs. Nature Communications, 8:15318.

42. Kun Li, Ting Li, Shan-shan Yang, Xu-De Wang, Lei-xin Gao, Rui-Qi Wang, JingGu, Xian-En Zhang*, Jiao-Yu Deng* (2017). Deletion of nudB causes increased susceptibility to antifolates in Escherichia coli and Salmonella enterica. Antimicrobial Agents & Chemotherapy, 61(5):02378-16.

43. Hua Li, Sha Wei, Yuan Fang, Min Li, Xia Li, Zhe Li, Jibin Zhang, Guofeng Zhu, Chuanyou Li, Lijun Bi, Guimin Zhang, Dianbing Wang,* and Xian-En Zhang* (2017).Quantitative proteomic analysis of host responses triggered by Mycobacterium tuberculosis infection in human macrophage cells. ABBS, 49 (9):835-844.

44. Zhitao Zhang, Dianbing Wang, Chuanyou Li, Jiaoyu Deng, Jibin Zhang, Lijun Bi and Xian-En Zhang* (2017). Microbial sensor for drug susceptibility testing of Mycobacterium tuberculosis. J Appl. Microbiol, 124(1): 286-293.

45. Jing Gu, Yuanyuan Chen, Hongsen Guo, Manluan Sun, Mingkun Yang, Xude Wang, Xian-En Zhang*, and Jiaoyu Deng* (2017). Lysine acetylation regulates the activity of Escherichia coil pyridoxine 5'-phosphate oxidase. ABBS, 49(2):186-192.

46. 张先恩* (2017). 科技强国植根于深厚的基础研究.中国科学院院刊, 32(5):496-503.

47. 张先恩* (2017). 生物传感发展50年及展望. 中国科学院院刊, 32(12):1271-1280.

48. 王殿冰, 崔宗强, 张先恩* (2017). 分子生物传感器与细胞内分子影像. 中国科学院院刊, 32(12):1303-1312.

49. 张先恩*, 刘云, 周程, 方在庆, 向桂林 (2017). 基础研究内涵及投入统计的国际比较. 中国软科学, (5):131-138.

二、非通讯作者论文（近三年发表）

1. Ali Ashaq, Noman Muhammad, Guo Yong, Liu Xu, Zhang Rong, Zhou Juan, Zheng Yang, Xian-En Zhang, Qi Yong*, Chen Xiaohua*, Men Dong* (2021). Myoglobin and C-reactive protein are efficient and reliable early predictors of COVID-19 associated mortality. Scientific Reports, 1(1), DOI: 10.1038/s41598-021-85426-9.

2. Weiwei Zhu, Ti Fang, Wenjing Zhang, Ao Liang, Hui Zhang, Zhiping Zhang, Xian-En Zhang, Feng Li* (2021). A ROS scavenging protein nanocage for in vitro and in vivo antioxidant treatment. Nanoscale, 13(8), 4634-4643.

3. Hongmei Shi, Ting Li, Jintian Xu, Jifang Yu, Shanshan Yang, Xian-En Zhang, Shengce Tao, Jing Gu*, Jiao-Yu Deng* (2021). MgrB Inactivation Confers Trimethoprim Resistance in Escherichia coli. Frontiers in Microbiology, (12), 6822085.

4. Wei Li, Ji Liu, Yuanyuan Liu, Qin Li, Wen Yin, Kevin K. Wanderi, Xiaowei Zhang, Zhiping Zhang, Xian-En Zhang, Zongqiang Cui* (2021). HIV-1 Uses Dynamic Podosomes for Entry into Macrophages. Journal of Virology, 95(10), e02480-20.

5. Yong Yang, Xi Wang, Ronghui Du, Wei Zhang, Haorui Si, Yan Zhu, Xurui, Shen, Qian Li, Bei Li, Dong Men, Yana Zhou, Hui Wang, Xiaolin Tong, Xian-En Zhang, Zhengli Shi, Peng Zhou* (2021). Serological investigation of asymptomatic cases of SARS-CoV-2 infection reveals weak and declining antibody responses. Emerging Microbes & Infections, 10(1), 905-912.

6. Minghai Chen, Siting Li, Wei Li, Zhiping Zhang, Xiaowei Zhang, Xian-En Zhang, Feng Ge*, Zongqiang Cui* (2021). Nanoscale Imaging of RNA-Protein Interactions with a Photoactivatable Trimolecular Fluorescence Complementation System. ACS Chemical Biology, 16(6), 1003-1010.

7. Wenjing Zhang, Qingyan Jia， Yibo Teng, Mengsi Yang, Hui Zhang, Xian-En Zhang, Pengfei Wang, Jiechao Ge*, Sheng Cao*, Feng Li* (2021). An Ultrastable Virus-Like Particle with a Carbon Dot Core and Expanded Sequence Plasticity. Small, 17(35), 2101717.

8. Ruimin Gao, Xian-En Zhang, Feng Li*(2021). Generation and characterization of self-assembled protein nanocages based on beta-carboxysomes in Escherichia coli. Acta Biochimica Et Biophysica Sinca, 53(7), 943-949.

9. Juan Zhang, Wenjing Zhang, Mengsi Yang, Weiwei Zhu, Ming Li, Ao Liang, Hui Zhang, Ti Fang, Xian-En Zhang, Feng Li*(2021). Passive cancer targeting with a viral nanoparticle depends on the stage of tumorigenesis. Nanoscale, 13(26), 11334-11342.

10. Yaguo Wang, Zihui Li, Shucai Wu, Fleming Joy, Chuanyou Li, Guofeng Zhu, Bo Chen, Baiguang Ren, Xingyun Wang, Boping Du, Pingjun Li, Peilei Hu, Juwang Yang, Yi Liu, Chongchen Zhou, Xian-En Zhang, Lijun Bi, Hongtai Zhang*, Junmei Yang*, Zongde Zhang*(2021). Systematic Evaluation of Mycobacterium tuberculosis Proteins for Antigenic Properties Identifies Rv1485 and Rv1705c as Potential Protective Subunit Vaccine Candidates. Infection and Immunity, 89(3). e00585-20.

11. Jie Gao, Li Zeng, Linlin Yao, Ziniu Wang, Xiaoxi Yang, Jinbo Shi, Ligang Hu, Qian Liu, Chunying Chen, Tian Xia, Guangbo Qu, Xian-En Zhang, Guibin Zhang* (2021). Inherited and acquired corona of coronavirus in the host: Inspiration from the biomolecular corona of nanoparticles. NANO TODAY, 39, 101161.

12. Xiao Wei Zhang, Hanzhong Wang, Yunan Sun, Mi Qi, Wei Li, Zhiping Zhang, Xian-En Zhang, Zongqiang Cui* (2020). Enterovirus A71 Oncolysis of Malignant Gliomas. Molecular Therapy, 28(6),1533-1546.

13. Xuehong Min, Ti, Fang, Lingling Li, Chaoqun Li, Zhiping Zhang, Xian-En Zhang, Feng Li* (2020). AIE nanodots scaffolded by mini-ferritin protein for cellular imaging and photodynamic therapy. Nanoscale,12(4),2340-2344.

14. Wen Yin, Wei Li, Qin Li, Yuanyuan Liu, Ji Liu, Min Ren, Yingxin Ma, Zhiping Zhang, Xiaowei Zhang, Yutao Wu, Shibo Jiang, Xian-En Zhang, Zongqiang Cui* (2020). Real-time imaging of individual virion-triggered cortical actin dynamics for human immunodeficiency virus entry into resting CD4 T cells. Nanoscale,12(1)115-129.

15. Yingxin Ma, Guobin Mao, Weiren Huang, Guoqiang Wu, Wen Yin, Xinghu Ji, Zishi Deng, Zhiming Cai, Xian-En Zhang, Zhike He, Zongqiang Cui* (2019). Quantum Dot Nanobeacons for Single RNA Labeling and Imaging. Journal of the American Chemical Society, 141(34):13454-13458.

16. Chong Qin, Wei Li, Qin Li, Wen Yin, Xiaowei Zhang, Zhi-Ping Zhang, Xian-En Zhang, Zongqiang Cui* (2019). Real-time dissection of dynamic uncoating of individual influenza viruses. PNAS, 116(7):2577-2582.

（资料来源：张先恩研究员，2021-12-27）